Purification, Cloning, and Expression of a Cytidine 5′-Monophosphate -Acetylneuraminic Acid Synthetase from
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چکیده
منابع مشابه
Mammalian cytidine 5'-monophosphate N-acetylneuraminic acid synthetase: a nuclear protein with evolutionarily conserved structural motifs.
Sialic acids of cell surface glycoproteins and glycolipids play a pivotal role in the structure and function of animal tissues. The pattern of cell surface sialylation is species- and tissue-specific, is highly regulated during embryonic development, and changes with stages of differentiation. A prerequisite for the synthesis of sialylated glycoconjugates is the activated sugar-nucleotide cytid...
متن کاملProduction and Purification of Secretory Simian Cytidine Monophosphate-N-acetylneuraminic Acid Hydroxylase Using Baculovirus-Protein Expression System.
Cytidine monophosphate (CMP) N-acetylneuraminic acid (Neu5Ac) hydroxylase (CMAH) is an essential enzyme for N-glycolylneuraminic acid (Neu5Gc) synthesis. In humans, Neu5Gc cannot be synthesized because of a deletion in the CMAH gene. Since Neu5Gc research has not been actively performed in comparison with Neu5Ac research, little is known about the function of Neu5Gc. Possible reasons are that C...
متن کاملEnzymatic synthesis of cytidine 5'-monophospho-N-acetylneuraminic acid.
We have established an efficient method for enzymatic production of cytidine 5'-monophospho-N-acetylneuraminic acid (CMP-NeuAc) from inexpensive materials, N-acetylglucosamine (GlcNAc) and cytidine 5'-monophosphate (CMP). The Haemophilus influenzae nanE gene encoding GlcNAc 6-phosphate (GlcNAc 6-P) 2-epimerase and the Campylobacter jejuni neuB1 gene encoding N-acetylneuraminic acid (NeuAc) synt...
متن کاملCloning, Expression, and Purification of Glutamine Synthetase from Clostridium acetobutylicum.
A glutamine synthetase (GS) gene, glnA, from the gram-positive obligate anaerobe Clostridium acetobutylicum was cloned on recombinant plasmid pHZ200 and enabled Escherichia coli glnA deletion mutants to utilize (NH(4))(2)SO(4) as a sole source of nitrogen. The cloned C. acetobutylicum gene was expressed from a regulatory region contained within the cloned DNA fragment. glnA expression was subje...
متن کاملThe sialic acids. X. Purification and properties of cytidine 5'-monophosphosialic acid synthetase.
An enzyme, cytidine monophosphate sialic acid synthetase, was purified from hog submaxillary glands. This enzyme (or enzymes) catalyzes the synthesis of CMP-N-acetylneuraminic acid or CMP-N-glycolylneuraminic acid or both and inorganic pyrophosphate from CTP and the corresponding sialic acids. The preparation was inactive with other known sialic acids and analogues of these compounds, and was a...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1996
ISSN: 0021-9258
DOI: 10.1074/jbc.271.26.15373